Drug aimed at 2 bioterror agents blocks live viral infection, Weill Cornell team reports
Dec 19, 2007 - 5:00:00 AM
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The next step, according to the researchers, is to use what they've learned to design even more effective peptides that should work even better.
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By New York- Presbyterian Hospital/Weill Cornell Medical Center/Weill Cornell Medical College,
[RxPG]
NEW YORK (Dec. 19, 2007) -- Two deadly and highly infectious viruses -- both potential bioterror threats -- may have met their match in a new drug developed by scientists at Weill Cornell Medical College in New York City.
Hendra and Nipah viruses are related, newly recognized zoonotic viruses that can spread from their natural reservoir in fruit bats to larger animals -- including pigs, horses and humans.
The mode of transmission isn't clear, but is thought to be relatively easy -- either by close contact with an infected host or by breathing in the microscopic pathogens. Infection often leads to a fatal encephalitis, and there is currently no effective treatment against these illnesses.
However, in breakthrough research conducted last year, researchers at Weill Cornell manipulated a peptide (protein) related to a third pathogen, parainfluenza virus, that appeared to block pseudo Hendra and Nipah viruses from entering and infecting human cells.
Now, this entry inhibitor approach has proven effective in blocking the infection of live virus in animal cells, pointing the way to a drug that could be stockpiled to help stop an outbreak in humans.
Those findings appeared recently in the Journal of Virology.
We have now tested the peptide-based entry inhibitor in monkey cells to show that it does effectively block infection with both live Hendra and Nipah, explains study senior researcher Dr. Anne Moscona, a professor of pediatrics and of microbiology and immunology at Weill Cornell Medical College, and an attending physician at NewYork-Presbyterian Hospital/Weill Cornell Medical Center.
Public health officials have sounded alarm bells ever since Nipah virus first emerged in pigs and then humans living in Southeast Asia. More recently, cases of Hendra virus began to show up in horses and their human handlers in Australia.
Experts who drew up the U.S. National Institute of Allergy and Infectious Diseases' Biodefense Research Agenda have included both viruses as potential bioterror agents.
Theoretically, it's possible to go out into the field and collect Hendra virus from bats, for example, Dr. Moscona says. We've been urgently working on this because right now there's absolutely nothing that can be done to stop this fatal, transmissible illness.
Luckily, prior research at Weill Cornell had laid out some important groundwork. The study's lead author, Dr. Matteo Porotto, has worked for years studying these types of microorganisms, using the parainfluenza virus as his model.
We were able to develop the strategy that we describe in this paper because our work on parainfluenza had already helped us understand how these viruses fuse with host cells, says Dr. Porotto, assistant professor of microbiology in the Department of Pediatrics at Weill Cornell Medical College.
Based on that work, Drs. Porotto and Moscona knew that when the receptor-binding molecule on the virus -- simply called G -- binds to the surface of the cell, it activates a special fusion protein. This fusion molecule has to then undergo some shape changes to turn itself into a six-helix bundle. Once that's done, it helps the virus fuse with, and enter, the cell, Dr. Porotto explains.
However, the Weill Cornell team discovered that a peptide specific to the parainfluenza virus fusion protein (F) can inhibit this shape-changing step -- stopping fusion cold.
Surprisingly, this parainfluenza F-peptide turned out to be even more effective at inhibiting Hendra virus fusion than peptides derived from the Hendra virus itself, Dr. Moscona says. It also appears to do much the same thing with the Nipah virus, inhibiting fusion there, too.
The team discovered just why the F peptide works so well in a collaboration with Dr. Min Lu, associate professor of biochemistry at Weill Cornell. These peptides act like door jambs -- their particular shapes prevent 'doors' in the viral 'fusion protein' from closing as they should. The parainfluenza peptide's shape simply makes it a better door jamb, Dr. Porotto said.
Much of this research is modeled on insights gained from two decades of investigation into another lethal virus, HIV. In fact, T-20, or Fuzeon -- one of the earliest effective HIV-suppressing drugs -- acts on a similar principle to block that virus' entry into cells.
The next step, according to the researchers, is to use what they've learned to design even more effective peptides that should work even better.
However, one issue with peptides is that you have to be concerned about how long they are going to last in the bloodstream, Dr. Moscona says. So, we are also developing methods of sustained-release -- for example, encasing the peptide in a polymer pellet that would be injected under the skin. The pellet would then release the drug slowly over the course of a week. That could form a viable method suitable for stockpiling, she says.
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