RxPG News Feed for RxPG News

Medical Research Health Special Topics World
 Asian Health
 Food & Nutrition
 Men's Health
 Mental Health
 Occupational Health
 Public Health
 Sleep Hygiene
 Women's Health
 Canada Healthcare
 China Healthcare
 India Healthcare
 New Zealand
 South Africa
 World Healthcare
   Latest Research
 Alternative Medicine
 Clinical Trials
 Infectious Diseases
 Sports Medicine
   Medical News
 Awards & Prizes
   Special Topics
 Odd Medical News

Last Updated: Oct 11, 2012 - 10:22:56 PM
Cytology Channel

subscribe to Cytology newsletter
Latest Research : Cytology

   EMAIL   |   PRINT
New method for the controlled initiation of membrane fusion

Oct 19, 2006 - 11:39:00 PM , Reviewed by: Priya Saxena
Using fast digital imaging, scientists from the Max Planck Institute of Colloids and Interfaces in Potsdam, Germany, together with researchers from Collège de France, have succeeded in developing two different protocols by which one can initiate the fusion process in a controlled manner and observe the subsequent fusion dynamics with a temporal resolution in the microsecond regime. For both protocols, the opening of the fusion necks was found to be very fast, with an average expansion velocity of centimetres per second. This velocity indicates that the initial formation of a single fusion neck can be completed in a few hundred nanoseconds.

[RxPG] The process of membrane fusion is essential for the structure and dynamics of all cells in our bodies. Fusion is indispensable for intracellular vesicle traffic, which sustains the compartmental organisation of cells. Likewise, membrane fusion is the basic molecular process that controls the communication between cells via the secretion of hormones, neurotransmitters, and growth factors. Furthermore, fusion processes are also crucial for the interactions between our cells and various pathogens such as viruses and bacteria. However, in spite of the ubiquity of membrane fusion, many aspects of this process have remained rather controversial. This situation reflects the absence of well-defined protocols by which one can induce fusion in a controlled manner and subsequently study its dynamics with high temporal resolution.

In order to clarify the dynamics of the fusion process in more detail, scientists from the Max Planck Institute of Colloids and Interfaces developed two different protocols for the fusion of unilamellar vesicles, which had a diameter of tens of micrometers but consist of only a single lipid membrane with a thickness of about four nanometers. Even though such a membrane is much thinner than the optical resolution limit, one can observe its shape using different methods of optical microscopy such as phase contrast and confocal microscopy, see Figure 1. The two protocols provide two different methods of bringing a pair of unilamellar vesicles into close contact, to initiate the fusion of their membranes in a controlled manner and to study the subsequent fusion dynamics with high temporal resolution.
New method for the controlled initiation of membrane fusion
Confocal microscopy images of lipid vesicles containing two different fluorescent dyes: (a) Two vesicles before fusion (equatorial section); (b) Vesicles fused by applying a short electric pulse; and (c) Three-dimensional image of a two-domain vesicle produced by fusion of two membranes with different composition (Image: Max Planck Institute of Colloids and Interfaces).

In the first protocol, artificial fusogenic molecules (a liposome whose outer wall contains molecules that cause cell fusion) or ligands, synthesized by the collaborators from Collège de France, were incorporated into the lipid membranes. Two unilamellar vesicles were aspirated by two glass micropipettes. Close proximity of the vesicle membranes was achieved by displacing these micropipettes. Membrane fusion was subsequently induced by the local addition of ions that form a complex between two fusogenic molecules embedded in the opposing membranes. In the second protocol, two lipid vesicles were brought into contact by alternating electric fields. Once close contact was established, membrane fusion was induced by exposing the vesicles to an additional electric pulse. Such a pulse leads to the formation of membrane pores in the opposing membranes, which subsequently fuse in order to dispose of the edges of the pores.

Both for ligand-mediated fusion and for electrofusion, the dynamics of fusion was observed using a fast digital camera with an acquisition rate of 20 000 frames per second, which corresponds to a temporal resolution of 50 microseconds. "Since previous direct imaging studies of membrane fusion were limited to time scales that exceed tens of milliseconds, the new experiments improved the temporal resolution by three orders of magnitude and revealed that the fusion process is surprisingly fast", says Rumiana Dimova, group leader in the Max Planck Institute of Colloids and Interfaces, and one of the participating scientists.

Indeed, only a few hundred microseconds after the initiation of the fusion process, the fusion neck connecting the two vesicles has already reached a diameter of a couple of micrometres as shown in Figure 1(b). This implies that the fusion neck has an average expansion velocity of centimetres per second and that the initial formation of the fusion neck can be completed within about 200 nanoseconds. This is in good agreement with recent computer simulations of tension-induced fusion. In this way, the Max Planck researchers have managed to bridge the gap between theoretical predictions and available experimental knowledge about the fusion process.

The experimental fusion protocols developed in the present study can be applied to other biomimetic systems and can be used to construct new ones. Particularly interesting systems, which can be studied in this way, are mixed membranes containing both lipids and fusogenic proteins such as SNAREs. One example for the construction of new biomimetic systems is provided by the formation of large vesicles with several intramembrane domains as shown in Figure 1(c). Another example consists of vesicles that contain different chemical reactants. The fusion of such vesicles initiates the corresponding chemical reactions in these rather small compartments and might be useful in order to synthesize new nanomaterials. In general, controlled membrane fusion has many potential applications in bioengineering, pharmacology, and medicine.

Publication: Proceedings of the National Academy of Sciences of the USA 103, 15841-15846, October 24, 2006
On the web: www.mpg.de 

Advertise in this space for $10 per month. Contact us today.

Related Cytology News
Ribosomes already showing medical importance
How cells adhere so firmly to blood vessel walls
New Insight into Cell Division
New method for the controlled initiation of membrane fusion
CPK3 and CPK6 function as ion channel regulators in guard cell signaling
Disrupted Intercellular Communication Causes a Disfiguring Birth Defect
Sharing Responsibility for Clathrin Coat Assembly
Understanding the process of AIF release following MOMP during apoptosis
Researchers discover new cell structures
Cilia also contribute to cellular response to external signals

Subscribe to Cytology Newsletter

Enter your email address:

 Additional information about the news article
Christopher K. Haluska, Karin A. Riske, Valérie Marchi-Artzner, Jean-Marie Lehn, Reinhard Lipowsky, and Rumiana Dimova
Timescales of membrane fusion revealed by direct imaging of vesicle fusion with high temporal resolution
Proc. Natl. Acad. Sci. USA 103, 15841-15846 (2006).
For any corrections of factual information, to contact the editors or to send any medical news or health news press releases, use feedback form

Top of Page

Contact us

RxPG Online



    Full Text RSS

© All rights reserved by RxPG Medical Solutions Private Limited (India)